Notice Correction RFA-DA-19-010 , Modeling HIV Neuropathology Using Microglia Human iPSC Cerebral Organoids R21 Clinical Trials Allowed) Notice Number: NOT-DA-19-010 Key Dates Release Date: November 15, 2018 Related Announcements RFA-DA-19-010 Issued National Institute Drug Abuse NIDA) Purpose reason this Notice to clarify purpose in RFA-DA-19-010, Modeling HIV Neuropathology Using Microglia Human iPSC Cerebral Organoids R21 Clinical Trial Allowed). Current Language: Part 2. Full Text Announcement Section I. Funding Opportunity Description Purpose Proposed projects MUST include following components. Applications lack three components be considered non-responsive the FOA will be reviewed.The major thrust the project MUST involve exploitation induced microglia cerebral organoids generated patient derived iPSC lines better understand molecular cellular mechanisms HIV-Associated Neurocognitive Disorder HAND). least aim sub-aim MUST also involve either 1. opioid, cannabinoid, methamphetamine, nicotinic, dopaminergic, other signaling pathways relevant addictive substance use, 2. exposure addictive substances, 3. analysis samples patients have used addictive substances have SUDs. Addictive substances interest include: opioids, nicotine, cocaine, methamphetamine, stimulants, prescription drugs, cannabinoids, alcohol, combinations these drugs. Applications focused solely alcohol exposure be considered non-responsive this FOA.” New Language: Part 2. Full Text Announcement Section I. Funding Opportunity Description Purpose Proposed projects MUST include of following components: 1) cerebral organoids containing microglia induced iPSCs; 2) cerebral organoids containing microglia induced iPSCs infected HIV; 3) cerebral organoids containing microglia induced iPSCs have exposed a drug abuse; 4) cerebral organoids containing microglia induced iPSCs have infected HIV exposed a drug abuse. iPSCs be derived normal health individuals, individuals affected a substance disorder SUD), individuals infected HIV; HIV infected individuals a SUD. Addictive substances interest include: opioids, nicotine, cocaine, methamphetamine, stimulants, prescription drugs, cannabinoids, alcohol, combinations these drugs. Applications focused solely alcohol exposure be considered non-responsive this FOA. HIV + HIV – Drug Abuse + Drug + HIV + Drug + HIV – Drug Abuse – Drug – HIV + Drug – HIV – Further Clarification: Investigators proposing create iPSC derived only cells individuals suffering a substance disorder are derived normal healthy individuals be considered responsive RFA-DA-19-009, Modeling HIV Neuropathology Using Microglia Human iPSC Cerebral Organoids R01 Clinical Trial Allowed) https://grants.nih.gov/grants/guide/rfa-files/RFA-DA-19-009.html , to accompanying RFA-DA-19-010 , Modeling HIV Neuropathology Using Microglia Human iPSC Cerebral Organoids R21 - Clinical Trials Allowed)https://grants.nih.gov/grants/guide/rfa-files/RFA-DA-19-010.html . Investigators still must create cerebral organoids containing microglia induced iPSCs are subsequently infected HIV. least aim must examine effect a drug abuse these organoids containing microglia the presence absence HIV infection. Applicants still propose derivation iPSC individuals a substance disorder individual infected HIV. other aspects this FOA remain same. Inquiries Please direct inquiries to: Da-Yu Wu, PhD National Institute Drug Abuse NIDA) Telephone: 301-435-4649 Email: wudy@mail.nih.gov